全文获取类型
收费全文 | 210篇 |
免费 | 51篇 |
国内免费 | 5篇 |
专业分类
儿科学 | 4篇 |
妇产科学 | 3篇 |
基础医学 | 7篇 |
临床医学 | 16篇 |
内科学 | 21篇 |
皮肤病学 | 1篇 |
神经病学 | 2篇 |
外科学 | 4篇 |
综合类 | 64篇 |
预防医学 | 74篇 |
眼科学 | 1篇 |
药学 | 50篇 |
1篇 | |
中国医学 | 6篇 |
肿瘤学 | 12篇 |
出版年
2024年 | 4篇 |
2023年 | 16篇 |
2022年 | 26篇 |
2021年 | 20篇 |
2020年 | 12篇 |
2019年 | 2篇 |
2018年 | 10篇 |
2017年 | 10篇 |
2016年 | 20篇 |
2015年 | 12篇 |
2014年 | 35篇 |
2013年 | 22篇 |
2012年 | 35篇 |
2011年 | 26篇 |
2010年 | 7篇 |
2009年 | 1篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 4篇 |
排序方式: 共有266条查询结果,搜索用时 15 毫秒
1.
目的 探讨低分子肝素治疗胎儿生长受限的效果及对胎儿窘迫发生率的影响.方法 选取2018年9月至2020年10月河北省唐山市妇幼保健院接受治疗,并诊断为胎儿生长受限的孕妇248例,根据随机数字表法分为对照组和观察组,各124例.对照组均接受常规基础治疗,观察组在对照组基础上同时接受低分子肝素治疗,7天一个疗程,1个疗程后休息7天,再进行下一个疗程,共治疗3个疗程.对比分析两组治疗前后双顶径、头围、宫高、腹围各项生长发育情况,治疗结束后,观察两组临床治疗有效率及胎儿窘迫、早产、新生儿窒息发生情况.结果 观察组胎儿双顶径、头围、宫高、腹围各项生长发育变化情况明显优于对照组(t=17.296、24.151、21.634、27.987,P均<0.05).观察组临床治疗有效率(97.58%)明显高于对照组(73.39%),差异有统计学意义(χ2=29.245,P<0.001).观察组胎儿窘迫、早产、新生儿窒息发生率均明显低于对照组(χ2=9.621、11.741、7.705,P均<0.05).结论 低分子肝素治疗胎儿生长受限临床疗效明显,能够显著降低胎儿窘迫等不良妊娠结局发生情况,促进胎儿生长发育. 相似文献
2.
3.
目的检测子痫前期病人胎盘组织、血清长链非编码 RNA H19(LncRNA H19)与长链非编码 RNAHAND2-AS1(Ln- cRNA HAND2-AS1)表达水平,并分析其与病人胰岛素抵抗( IR)的相关性。方法将 2021年 1—12月在唐山市妇幼保健院建卡的子痫前期孕妇 105例作为子痫前期组,根据子痫前期孕妇严重程度分为轻度子痫前期组与重度子痫前期组,另选取同期孕周、年龄相符的健康孕妇 97例作为对照组,收集所有孕妇身体质量指数(BMI)、孕周、收缩压、舒张压、空腹胰岛素(FINS)、空腹血糖( FBG)等一般资料,并计算稳态模型的 IR指数( HOMA-IR);实时荧光定量 PCR(qRT-PCR)法测定孕妇血清、胎盘组织中 LncRNA H19,LncRNA HAND2-AS1水平;比较对照组与子痫前期组、轻度子痫前期组与重度子痫前期组间血清、胎盘组织 LncRNA H19、LncRNA HAND2-AS1水平; Pearson法分析子痫前期孕妇血清、胎盘组织 LncRNA H19、LncRNA HAND2-AS1与 HOMA-IR的相关性。结果子痫前期组病人收缩压、舒张压高于对照组(P<0.05)且重度子痫前期病人收缩压、舒张压高于轻度子痫前期病人,孕周低于轻度子痫前期病人(P<0.05);与对照组相比,子痫前期组孕,妇 24 h尿蛋白、 FBG[(5.84±0.97)mmol/L比(4.22±0.70)mmol/L]、FINS[(13.37±2.23)mU/L比( 7.52±1.25)mU/L]、HOMI-IR水平(3.47±0.57比 1.41±0.23)升高(P<0.05),且重度子痫前期孕妇 24 h尿蛋白、 FBG[(6.90±1.15)mmol/L比( 5.24±0.85)mmol/L]、 FINS[(13.97±2.32)mU/L比( 13.05±2.17)mU/L]、 HOMI-IR水平( 4.27±0.71比 3.03±0.50)高于轻度子痫前期孕妇( P<0.05);与对照组相比,子痫前期组孕妇血清、胎盘组织 Ln- cRNA H19(2.52±1.42比 1.01±0.15,3.75±0.62比 1.02±0.17)与 LncRNA HAND2-AS1水平( 1.98±0.33比 1.01±0.15,2.87±0.47比1.02±0.17)升高( P<0.05)且重度子痫前期孕妇血清、胎盘组织 LncRNA H19(2.84±0.47比 2.34±0.39,4.28±0.71比 3.45±0.57)与LncRNA HAND2-AS1水平(,2.59±0.43比 1.63±0.27,3.56±0.59比 2.49±0.41)高于轻度子痫前期孕妇( P<0.05);子痫前期病人血清与胎盘组织间 LncRNA H19水平呈正相关( r=0.55,P<0.05)血清与胎盘组织间 LncRNA HAND2-AS1水平呈正相关性( r=0.65,P<0.05)。子痫前期孕妇血清、胎盘组织 LncRNA H19水别与 HOMI-IR呈正相关( r=0.53、0.59,P<0.05);血清、胎盘组织 LncRNA HAND2-AS1水平分别与 HOMI-IR呈正相关( r=0.60、0.61,P<0.05)。结论子痫前期病人血清、胎盘组织 LncRNA 平分,H19、LncRNA HAND2-AS1高表达,二者与 IR密切相关,可能通过影响 IR参与子痫前期发生发展。 相似文献
4.
目的研究超声评分系统、螺旋CT及血清学指标在鉴别青春期卵巢肿瘤中的价值。方法将医院2016年1月至2018年1月间,96例经手术病理确诊为青春期卵巢肿瘤患者纳为研究对象,以手术病理结果为“金标准”,研究超声评分系统、螺旋CT及血清学指标在诊断青春期良恶性卵巢肿瘤中的价值。结果96例患者全部行手术治疗,术中冰冻检查提示良性61例,恶性35例,青春期卵巢恶性肿瘤以生殖细胞肿瘤为主的有45.71%(16/35);共91例患者术前行彩色多普勒超声检查,Alaczar评分系统在鉴别青春期卵巢肿瘤良恶性中的灵敏度、特异度及准确度分别为91.23%、91.18%和91.21%;共20例患者术前行螺旋CT检查,螺旋CT在鉴别青春期卵巢肿瘤中的灵敏度、特异度及准确度分别为75.00%、93.75%和90.00%;所有患者术前均行血清学检查,检查结果提示血清癌胚抗原125(CA125)水平异常者66例(44~569.45U/mL),术后病理检查提示良性病变51例,恶性病变15例;血清糖链抗原199(CA199)水平异常者26例(46~151U/mL),其中良性病变7例,恶性病变19例;血清甲胎蛋白(AFP)水平异常者16例(215.15~3751.21ng/mL),病理检查提示恶性病变者9例,良性病变者7例。结论青春期卵巢肿瘤发病隐匿,临床表现缺乏特异性,需医者仔细检查,结合各种信息加以判断,而超声评分系统、螺旋CT及血清肿瘤标志物在辅助鉴别青春期卵巢肿瘤良恶性中均具有一定效能。 相似文献
5.
6.
摘 要 目的: 建立蒲药灌肠液中香蒲新苷、异鼠李素-3-O-新橙皮苷和延胡索乙素的HPLC含量测定方法。方法: 采用ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.1%磷酸(三乙胺调节pH至6.0)为流动相,梯度洗脱程序,流速:1.0 ml·min-1,检测波长为254 nm(0~14 min)和281 nm(14~25 min),柱温:30 ℃。结果: 香蒲新苷、异鼠李素-3-O-新橙皮苷和延胡索乙素的线性范围分别为19.840~198.400 μg·ml-1(r=0.999 6)、20.520~205.200 μg·ml-1(r=0.999 8)和10.040~100.400 μg·ml-1(r=0.999 7),回收率分别为98.8%、98.6%和98.9%,RSD分别为1.4%、1.6%和1.3%(n=6)。结论: 该方法灵敏度高,专属性强,可用于蒲药灌肠液的质量控制。 相似文献
7.
Gastrodin has been showed to possess many beneficial physiological functions, including protection against inflammation and oxidation and apoptosis. Studies showed inflammation and oxidation play important roles in producing liver damage and initiating hepatic fibrogenesis. However, it has not been reported whether gastrodin has a protective effect against hepatic fibrosis or not. This is first ever made attempts to test gastrodin against liver fibrosis in bile duct ligation (BDL) rats. The aim of the present study is to evaluate the effect of gastrodin on BDL-induced hepatic fibrosis in rats. BDL rats were divided into two groups, BDL alone group, and BDL-gastrodin group treated with gastrodin (5 mg/ml in drinking water). The effects of gastrodin on BDL-induced hepatic injury and fibrosis in rats were estimated by assessing serum, urine, bile and liver tissue biochemistry followed by liver histopathology (using hematoxylin & eosin and sirius red stain) and hydroxyproline content measurement. The results showed that gastrodin treatment significantly reduced collagen content, bile duct proliferation and parenchymal necrosis after BDL. The serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) decreased with gastrodin treatment by 15.1 and 23.6 percent respectively in comparison to BDL group did not receive gastrodin. Gastrodin also significantly increased the level of serum high density lipoprotein (HDL) by 62.5 percent and down-regulated the elevated urine total bilirubin (TBIL) by 56.5 percent, but had no effect on total bile acid (TBA) in serum, bile and liver tissues. The immunohistochemical assay showed gastrodin remarkably reduced the expressions of CD68 and NF-κB in BDL rats. Hepatic SOD levels, depressed by BDL, were also increased by gastrodin by 8.4 percent. In addition, the increases of hepatic MDA and NO levels in BDL rats were attenuated by gastrodin by 31.3 and 38.7 percent separately. Our results indicate that gastrodin significantly attenuated the severity of BDL-induced hepatic injury and fibrosis by attenuating oxidative stress and inflammation. Taken together, these findings suggest that gastrodin might be an effective antifibrotic drug in cholestatic liver disease. 相似文献
8.
目的:探讨小儿反复呼吸道感染(RRTI)的危险因素,为降低儿童RRTI患病率制定相应的预防措施。方法:采用成组设计的病例对照研究方法,对RRTI病例及健康小儿各101例进行问卷调查,采用原子吸收法检测血微量元素(血锌、铜、铁、钙、镁)及血铅水平,采用免疫透射比浊法测定IgM、IgA、IgG、补体C3、补体C4的含量。应用Logistic回归模型控制混杂因素干扰。结果:儿童期不偏食、血锌为保护性因素。血铅含量偏高、主要看护人不是自己父母、户外活动时间短、既往佝偻病史为RRTI的可能危险因素。结论:运用科学的营养方式,杜绝偏食,坚持体育锻炼及户外活动,父母多看护儿女,定期检测微量元素,必要时补充锌或驱铅治疗,提高机体抵抗力,将会减少RRTI发生率。 相似文献
9.
10.
《Vaccine》2018,36(9):1167-1173
Although Enterovirus 71 (EV71) has only one serotype based on serum neutralization tests using hyperimmune animal antisera, three major genogroups (A, B and C) including eleven genotypes (A, B1-B2, and C1-C5) can be well classified based on phylogenetic analysis. Since 1997, large-scale EV71 epidemics occurred cyclically with different genotypes in the Asia-Pacific region. Therefore, development of EV71 vaccines is a national priority in several Asian countries. Currently, five vaccine candidates have been evaluated in clinical trials in China (three C4 candidates), Singapore (one B2 candidate), and Taiwan (one B4 candidate). Overall, the peak viral titers of these 5 vaccine candidates could only reach about 107 TCID50/mL. Moreover, genotypes of these 5 candidates are different from the current predominant genotype B5 in Taiwan and South-Eastern Asia. We adapted a high-growth EV71 genotype B5 (HG-B5) virus after multiple passages and plaque selections in Vero cells and the HG-B5 virus could reach high titers (>108 TCID50/mL) in a microcarrier-based cell culture system. The viral particles were further purified and formulated with alum adjuvant. After two doses of intramuscular immunization in rabbits, the HG-B5 vaccine candidate could induce cross-reactive neutralizing antibodies against the three major EV71 genogroups. In conclusion, a high-growth EV71 virus was successfully adapted in Vero cells and could induce broad spectrum neutralizing antibody titers against three (A, B5, and C4) genotypes in rabbits. 相似文献